One thought on “NHS MANCHESTER FOI request shows NHS are using cycles at 45 which is medical fraud & 97% false positive.”
I believe the clerk who answered the FOI request has mixed up cycles with cycle thresholds. They run the PCR to 45 cycles, but their cycle threshold (Ct) cutoff to determine positive/negative is 40 cycles (which is of course still too high and doesn’t correspond to active infection or infectiousness). The Ct line is placed in the exponential phase of PCR amplification, above background noise. To find the exponential phase of the growth curve of the PCR product, you have to run the PCR past that phase until you reach the plateau. See page 5 of the following NHS document for the Ct cutoff of 40 cycles, and page 16 shows that they run the PCR essay for 45 cycles.
Figure 18 on page 33/34 of the following US CDC document illustrates the placement of Ct in the exponential growth phase of the PCR product. https://www.fda.gov/media/134922/download
Rights and Freedoms
I believe the clerk who answered the FOI request has mixed up cycles with cycle thresholds. They run the PCR to 45 cycles, but their cycle threshold (Ct) cutoff to determine positive/negative is 40 cycles (which is of course still too high and doesn’t correspond to active infection or infectiousness). The Ct line is placed in the exponential phase of PCR amplification, above background noise. To find the exponential phase of the growth curve of the PCR product, you have to run the PCR past that phase until you reach the plateau. See page 5 of the following NHS document for the Ct cutoff of 40 cycles, and page 16 shows that they run the PCR essay for 45 cycles.
Click to access Guidance-and-SOP-COVID-19-Testing-NHS-Laboratories.pdf
Figure 18 on page 33/34 of the following US CDC document illustrates the placement of Ct in the exponential growth phase of the PCR product.
https://www.fda.gov/media/134922/download
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